Unlock the Future of Beauty & Health.
High-Quality Human Stem Cell Conditioned Media.
"Human stem cell conditioned media" has recently attracted significant attention in the fields of regenerative medicine and beauty. Alpha International Communications LLC is pleased to announce the launch of high-quality, freeze-dried (FD) human stem cell conditioned media products [adipose-derived / tooth pulp-derived / umbilical cord-derived] manufactured by Pure Stem. These products are created using stem cells collected from healthy Japanese donors selected under strict criteria. The cells are cultured at the Osaka Regenerative Medicine Center, a domestic Cell Processing Center (CPC), and then processed using a specialized freeze-drying technology. Containing high concentrations of abundant cytokines (growth factors) and exosomes, and having passed rigorous safety tests, it is a highly reliable cosmetic ingredient.
What is Human Stem Cell Conditioned Media?
Stem Cells and Conditioned Media:
Stem Cells: These are unique cells characterized by their ability to differentiate into various cell types (differentiation potential) and to replicate themselves (self-renewal).
Stem Cell Conditioned Media (Supernatant): This is the supernatant portion of the culture medium in which stem cells have been cultured. It is abundant in various bioactive substances secreted by the stem cells, such as cytokines, exosomes, and enzymes. Importantly, it does not contain the stem cells themselves, drawing attention for its ethical profile and safety.
Potential Benefits:
- The hundreds of types of cytokines and exosomes within the conditioned media are believed to function as intercellular messengers, supporting processes such as cell activation, tissue repair, and anti-inflammatory actions.
- In the cosmetics field, its potential applications include promoting skin turnover, enhancing firmness and elasticity through support for collagen and elastin production, improving the appearance of dark spots and wrinkles, aiding wound healing, and stimulating hair growth.
The Pure Stem Difference:
Features and Commitment of Human Stem Cell Conditioned Media FD
1. Carefully Selected High-Quality "Donors" and "Source Cells":
- Adipose-derived:
- Collected from adipose tissue of healthy Japanese donors in their 20s and 30s.
- Culture initiated within 24 hours of collection.
- Tooth Pulp-derived:
- Collected from deciduous tooth pulp of healthy Japanese donors aged 6-8.
- Culture initiated within 24 hours of collection. Cells from young donors are considered highly vital.
- Umbilical Cord-derived (Wharton's Jelly):
- Collected from the Wharton's jelly of umbilical cords from healthy Japanese donors in their 20s and 30s.
- Culture initiated within 36 hours of collection.
- Umbilical cords are rich in mesenchymal stem cells (MSCs) with high multipotency.
- Donor Screening: All donors are confirmed healthy and free from viral infections (including HBV, HCV, HIV, HTLV, etc.; Hepatitis E is also tested for adipose and tooth pulp donors, while Syphilis and Parvovirus B19 are tested for umbilical cord donors) through medical interviews and blood tests prior to collection.
2. Abundant "Cytokines" and "Exosomes":
- Our proprietary culture technology yields high concentrations of various cytokines. The content is strictly measured and managed for each lot (see product-specific information for details).
- Also rich in "exosomes," considered key mediators of intercellular communication. (Example: Adipose-derived Rich: 7.3 billion/vial, Tooth Pulp-derived Rich: 14.1 billion/vial)
3. Thorough "Safety" Management:
- Domestic Manufacturing: Produced under a strict management system at the Osaka Regenerative Medicine Center (CPC).
- Serum-Free Medium: Utilizes safe, serum-free culture medium.
- Various Safety Tests: Confirmed negative results for sterility, mycoplasma, and endotoxin tests conducted according to methods compliant with the Japanese Pharmacopoeia.
- Virus Testing: In addition to donor screening, the conditioned media undergoes virus testing (HIV-1, HIV-2, HBV, HCV, HTLV) with confirmed negative results.
- Raw Material Standards: Confirmed that heavy metals (including arsenic and cadmium), viable bacteria, etc., are below detectable limits.
- Virus-Free Certificate: A certificate proving each lot is virus-free is issued.
4. Special "Freeze-Drying (FD)" Technology:
A special technique where the conditioned media is frozen, and water content is sublimated under vacuum.
- Benefits:
- Convenience for Use: Allows for reconstitution with a solvent (e.g., purified water) immediately before use, ensuring freshness.
- Quality Preservation: Minimizes denaturation and inactivation of heat-sensitive bioactive substances like cytokines and exosomes, maintaining high activity.
- Long-Term Stability: Being free of water reduces risks of microbial contamination and chemical changes, enabling stable quality for extended periods (2 years post-shipment).
- Ease of Transport & Storage: Lightweight and compact. Can be stored at room temperature and is easy to manage.
- Preservative-Free: Enhanced preservation through freeze-drying eliminates the need for added preservatives.
Product Lineup (Adipose / Tooth Pulp / Umbilical Cord)
▼ The Potential of Pure Stem Human Stem Cell Conditioned Media ▼
Why is human stem cell conditioned media garnering so much attention now?
Beyond its approach to skin, the reason lies in the diverse functions of the bioactive substances it contains, such as cytokines and exosomes. These components are reported in numerous studies to play crucial roles related to tissue repair and maintaining healthy balance within the body. In addition to applications in the beauty and anti-aging fields, recent research is exploring perspectives like daily vitality support and contribution to QOL (Quality of Life) improvement, generating high expectations for its potential.
Human Adipose-Derived Stem Cell Conditioned Media FD Rich
▼ For a Multi-Faceted Approach to Signs of Aging ▼
Total anti-aging care leading to vibrant, confident skin. "Human Adipose-Derived Stem Cell Conditioned Media" is effective as a cosmetic ingredient to address such concerns.
Origin:
Adipose tissue from healthy Japanese donors in their 20s and 30s.
Features:
Relatively easy to collect and extensively researched.
Contains cytokines anticipated for broad applications such as supporting skin firmness/elasticity, wound healing, and hair growth.
Product Cytokine Specifications:
- TGF-β: 253−283 pg/mL or higher
- (Noted for its role in supporting skin firmness and elasticity through promoting collagen/elastin production and wound healing.)
- VEGF: 38.3−70.8 pg/mL or higher
- (Supports healthy microcirculation for a vibrant skin appearance, linked to functions like angiogenesis, wound healing, and hair growth support.)
- EGF: 21.4−25.3 pg/mL or higher
- (Supports skin turnover by promoting epidermal cell proliferation, leading to smoother skin.)
Exosome Count (Rich): 7.3 billion particles/Vial
Safety: Complies with Virus-Free Certificate and Raw Material Standard Specification.
PureStem Human Tooth Pulp Stem Cell Conditioned Media FD Rich
▼ Aiming for Clear Radiance and a Youthful Impression ▼
Advanced skincare and scalp care designed to bring out the skin's inherent clarity and radiance. "Human Tooth Pulp-Derived Stem Cell Conditioned Media" is a cosmetic ingredient derived from young donors.
Origin:
Deciduous tooth pulp from healthy Japanese donors aged 6-8.
Features:
Derived from young donors, whose cells are considered highly active. Contains nerve growth factor (NGF), vascular endothelial growth factor (VEGF), and hepatocyte growth factor (HGF). Research is exploring its applications related to neuroprotection, tissue regeneration, and skin brightening.
Product Cytokine Specifications:
- β-NGF: 2.5−3.0 pg/mL or higher
- (Expected to help maintain healthy skin, as it is believed to be involved in nervous system support, including nerve cell growth, maintenance, repair, and wound healing.)
- VEGF: 153−213 pg/mL or higher
- (Supports healthy microcirculation for a vibrant skin appearance, linked to functions like angiogenesis, wound healing, and hair growth support.)
- HGF: 155−185 pg/mL or higher
- (Known to be involved in creating a clear skin impression by conditioning the skin environment, related to cell proliferation/migration, tissue repair, skin brightening, and hair growth support.)
Exosome Count (Rich): 14.1 billion particles/Vial
Safety: Complies with Virus-Free Certificate and Raw Material Standard Specification.
PureStem Human Umbilical Cord Stem Cell Conditioned Media FD Rich
▼ Powerful Approach for Complex Concerns ▼
Pinnacle anti-aging care aiming for next-level beauty by addressing various signs of aging. "Human Umbilical Cord-Derived Stem Cell Conditioned Media" is a cosmetic ingredient derived from the umbilical cord, the source of life's beginning.
Origin:
Wharton's jelly from umbilical cords of healthy Japanese donors in their 20s and 30s.
Features:
Contains a diverse range of cytokines, with particularly high concentrations of HGF, EGF, and KGF. Derived from mesenchymal stem cells (MSCs) with high multipotency. Highly researched in the regenerative medicine field, with expectations for its anti-inflammatory, immunomodulatory, and potent tissue repair capabilities. Anticipated to benefit overall anti-aging care.
Key Contained Cytokines:
- HGF: 3500 pg/mL or higher
- (Known to condition the skin environment for a clear skin impression, related to functions like cell proliferation/migration, tissue repair, skin brightening, and hair growth support.)
- EGF: 250 pg/mL or higher
- (Supports skin turnover by promoting epidermal cell proliferation, leading to smoother skin.)
- KGF: 20 pg/mL or higher
- (Related to epidermal cell proliferation, barrier function, wound healing, and hair growth support.)
Exosome Count (Rich): 14.1 billion particles/Vial
Safety: Complies with Virus-Free Certificate and Raw Material Standard Specification.
Key Differentiators from General Products
- High-Quality Raw Materials:
- Sourced exclusively from young, healthy Japanese donors with strict control over post-collection processing time.
- High Concentration:
- Proprietary culture technology and freeze-drying process ensure high concentration and activity of cytokines and exosomes.
- Domestic Manufacturing & Control:
- High reliability through manufacturing at a domestic CPC (Osaka Regenerative Medicine Center) and rigorous quality/safety testing.
- Clear Information Disclosure:
- Disclosure of donor information, cytokine content, safety test results, etc.
- Freeze-Drying:
- Suppresses quality degradation, enables long-term stable storage. Preservative-free.
Other Selling Points
- Traceability:
- Ensured traceability of raw materials via donor identification numbers.
- Three Origins:
- Allows selection of the optimal origin (Adipose/Tooth Pulp/Umbilical Cord) according to product concept and target audience.
Contact Us
For product details, quotations, sample requests, technical questions, or any other inquiries, please feel free to contact us.
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